Effects of Benzene on Kidney Structure in Mice (Albino Nmri)

Purpose: Study of benzene effects on kidney structure in mammals (mouse). Materials and Methods: The mice were studied in 3 groups: controls, solvent and treatment, at favorable laboratory conditions. Treatment mice were administered orally of benzene (0.1cc of benzene solution) for 10 days at a dose 1000 mg/kg of body weight. At the end of each period, the kidneys separated and fixed in paraffin 10% and this sections (6 micron diameter) were prepared by microtome. Then data were analyzed by ANOV A and t-test methods. Results: Microscopic and statistical studies made on the selected mice, showed that: in the treatment groups, the kidney was changed to an congestion organ. There are some significant changes in the proportion of cortical, outer and inner medulla layer, proximal, distal, henle cellular wall's thickness, malpighian corpuscle space, kidney texture and finally these events can be in relation with poisoning effects of benzene on natural renal. Conclusion: Since in the formation of urine, proportional spread of blood vessels in primary glomerular plexus at malpighian corpuscles and in peritubular glomerulus's plexus is very important; therefore abnormal changes in cortical, medulla thickness of kidney's tubules and some events such as: nephrite, development of capillary plexus, dilatation of bowman's capsule space, even presence of blood plasma in tubular lumen and decrease of tubular cell wall diameter in treatment sample, indicate that benzene has considerable effects on structure and function of different parts of kidney.